The high performance liquid chromatography Diaries
The high performance liquid chromatography Diaries
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ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
The cell phase’s movement charge is set with the combined speeds of the two pumps. By shifting the relative speeds of the two pumps, distinct binary mobile phases is often geared up.
A different handy detector is a mass spectrometer. Figure twelve.five.thirteen reveals a block diagram of a standard HPLC–MS instrument. The effluent within the column enters the mass spectrometer’s ion source employing an interface the removes a lot of the cell phase, an essential require due to incompatibility in between the liquid cell phase and the mass spectrometer’s high vacuum atmosphere.
物質の電気化学的な性質を利用した検出器。pHの変動や酸化還元電位の変動を用いて測定を行う。
. Example of a typical high-performance liquid chromatograph with insets displaying the pumps that transfer the cell phase from the system as well as the plumbing used to inject the sample in to the cell stage.
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-hydroxybenzoic acid (PH) with a nonpolar C18 column topic to a greatest Examination time of 6 min. The shaded areas characterize locations wherever a separation is impossible, Together with the unresolved solutes recognized.
It achieves this by exploiting the differing interactions of sample compounds with two critical phases: the cellular section and the stationary period. Comprehension the Main elements of an HPLC system as well as their roles is essential for thriving analysis.
-hydroxybenzoic acid—with a nonpolar C18 column applying an aqueous buffer of acetic acid and sodium acetate because the cell section. The retention periods for these weak acids are shorter when utilizing a much less acidic cellular get more info phase for the reason that Just about every solute is current in an anionic, weak base type that is certainly a lot less soluble during the nonpolar stationary section.
In this certain instrument, Each and every pump sends its cell phase to the mixing chamber where they Merge to sort the ultimate mobile stage. The relative speed of the two pumps decides the cell phase’s ultimate composition.
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The choice to get started with acetonitrile is arbitrary—we will just as easily opt for to start with methanol or with tetrahydrofuran.
특히 컬럼의 선정은 분석의 결과에 영향을 미치기에 신중하게 선택하여야 합니다.
. Illustration of a normal high-performance liquid chromatograph with insets exhibiting the pumps that go the mobile stage website with the system as well as plumbing accustomed to inject the sample to the cell section.